LOCUS       pLV-[hsa-miR-1270-2]        9137 bp    DNA     circular     2-MAR-2011
SOURCE      
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7058..7918
                     /label=Amp(R)
     promoter        8119..8659
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5663..5897
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6472..6927)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5969..6099
                     /label=SV40 pA
     misc_feature    6100..6313
                     /label=SV40 ori
     misc_feature    4984..5580
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3602
                     /label=intron
     CDS             3649..4974
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3392
                     /label=hsa-miR-1270-2 precursor
BASE COUNT     2171 a      2366 c      2458 g      2142 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct gaactcctga 
     3121 cctcaagtga ttcacccaca tctgcctccc aaagtgttgg gtttacagac acaagccacc 
     3181 acaccaggcc aataatttac ttttaaaggc acagagttat actggagata tggaagagct 
     3241 gtgttgggta taagtaacag gcttttcttt atcttctatg tggctctttg cattgtgctc 
     3301 acctgggacc cttcacacac ttaattcact tatacatttt ttacaaattg tattttggtc 
     3361 agtatgtttt tgttacattt atatgtccag gagctagctc gagcttttgg agtacgtcgt 
     3421 ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga 
     3481 ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt 
     3541 ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc 
     3601 aggtgtcgtg aaaactaccc ctctagagtc gagctaccgg tcgccaccat ggtgagcaag 
     3661 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 
     3721 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 
     3781 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 
     3841 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 
     3901 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 
     3961 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 
     4021 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 
     4081 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 
     4141 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 
     4201 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 
     4261 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 
     4321 cgccactcca ccggcggcat ggacgagctg tacaaggacc caccggtcgc caccatgacc 
     4381 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc 
     4441 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac 
     4501 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc 
     4561 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc 
     4621 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg 
     4681 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg 
     4741 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc 
     4801 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc 
     4861 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc 
     4921 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgagcggcc 
     4981 gcgtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact 
     5041 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg 
     5101 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg 
     5161 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa 
     5221 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc 
     5281 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg 
     5341 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat 
     5401 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt 
     5461 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc 
     5521 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg 
     5581 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca 
     5641 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct 
     5701 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg 
     5761 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt 
     5821 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt 
     5881 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc 
     5941 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa 
     6001 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 
     6061 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta 
     6121 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     6181 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag 
     6241 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt 
     6301 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta 
     6361 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg 
     6421 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct 
     6481 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 
     6541 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 
     6601 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 
     6661 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct 
     6721 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 
     6781 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt 
     6841 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 
     6901 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac 
     6961 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc 
     7021 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt 
     7081 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct 
     7141 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga 
     7201 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag 
     7261 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca 
     7321 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga 
     7381 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag 
     7441 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc 
     7501 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa 
     7561 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt 
     7621 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg 
     7681 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt 
     7741 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg 
     7801 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat 
     7861 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact 
     7921 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa 
     7981 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt 
     8041 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt 
     8101 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg 
     8161 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca 
     8221 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt 
     8281 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga 
     8341 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc 
     8401 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact 
     8461 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga 
     8521 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg 
     8581 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt 
     8641 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt 
     8701 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga 
     8761 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac 
     8821 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc 
     8881 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 
     8941 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc 
     9001 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 
     9061 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga 
     9121 acaaaagctg gagctgc 
//
